The genotyping of ABO-blood group by PCR-RFLP method.
نویسندگان
چکیده
منابع مشابه
[ABO genotyping by PCR-direct sequencing method].
OBJECTIVE To analyze the sequence difference between human A, B, and O alleles and establish the method of ABO genotyping by PCR direct sequencing. METHODS PCR-direct sequencing technique was used to analyze two regions of cDNA from A transferase gene, 233-433 and 660-788. RESULTS Two nucleotide substitutions at 258th and 297th were found in 233-433 region, and a nucleotide substitution at ...
متن کاملBlood Group ABO Genotyping in Paternity Testing.
BACKGROUND: The ABO blood groups result from DNA sequence variations, predominantly single nucleotide and insertion/deletion polymorphisms (SNPs and indels), in the ABO gene encoding a glycosyltransferase. The ABO blood groups A(1), A(2), B and O predominantly result from the wild type allele A1 and the major gene variants that are characterized by four diallelic markers (261G>del, 802G>A, 803G...
متن کاملGenotyping of samples lacking expected antibodies in ABO blood group.
We report nine donations with ABO inconsistency in reverse typing caused by partly or entirely missing antibodies. A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence-specific priming and sequencing. A B101 allele was demonstrable in a case with O phenotype. The molecular mechanisms in deficiency of n...
متن کاملThe effect of parental ABO blood group on fetal surveillance
Background Several factors may cause infertility and fetal loss. Blood groups antigens seem to be implied in the mechanisms of infertility and fetal loss. Maternal natural antibody can react against father’s blood group antigens on spermatozoa. The effects of parental blood group system on infertility and fetal surveillance perceived by its manifestation in prezygotic (caused infertility) and ...
متن کاملABO genotyping in Italian blood donors.
BACKGROUND Traditional ABO blood group serology is based on the immunoreactivity of antisera with the carbohydrate A, B and H antigens. Progress in the molecular biology of the ABO system has recognized the molecular basis of the red cell (RBC) antigens and has provided a genetic model for ABO polymorphism at the molecular level. Recently, this genetic model was tested in a large number of indi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the Japan Society of Blood Transfusion
سال: 1993
ISSN: 1883-8383,0546-1448
DOI: 10.3925/jjtc1958.39.575